بررسی نقش کلیستوتسیوم درزمستان گذرانی Uncinula necator (Schw.)Burr. عامل بیماری سفیدک سطحی انگور در استان خراسان

A 2-year vineyard survey failed to the provide evidence that Uncinula necator (Schw.) Burr. survived winter as mycelium in dormant infected buds in Khorassan province. Ascospores of U. necator, were collected by a volumetric spore trap operating constantly in a vineyard for 55 days after the bud burst. The first powdery mildew colonies were consistently found on the leaves of the shoots (7.30 cm long) growing on the vine. Cleistothecia were found on all plant parts infected during the previous growing season. More than 35-45 % of the cleistothecia borne on the leaves and stems died during winter. Most of the ascospore discharge occurred between the bud burst and the blooming time. Ascospores were periodically released from cleistothecia on the leaves kept in vineyard from October to May, while the ascospores germinating on the glass slides germinated from October to January and then germination slowed down to zero in early March and the water content potential of ascospore cytoplasm decreased constantly during this period as well. The mass required to fracture the ascocarp wall during maturation was measured to be approximately 5g in autumn, 3g in winter and 2.5 g in early spring. The most rapid decrease in the strength of the ascocarp wall occurred during a 4-week period before dehiscence in the field. The effect of the pathogenic property ascospores on healthy leaves demonstrated their role as a primary inoculum source. Cleistothecia appear to be the principal means of overwintering of U. necator in Khorassan province vineyards.